1. Annotated regions used for antigen quantification are shown for a representative lymph node.
  2. Segment type is shown below for a representative lymph node.
  3. Antigen signal is shown below for a representative lymph node.
  4. 21 day Ag signal is shown for Lyve1+ and CD11C+ segments from mice that received a single 21 day vaccination and mice that received sequential 21/42 day vaccinations. The 42 day samples were excluded due to the low signal observed at this later timepoint. The number of segments plotted is shown above each boxplot.
  5. Ag signal is shown for Lyve1+ and CD11C+ segments. Note the strong correlation between Ag signal for Lyve1+ and CD11C+ segments.


Extended figures

The sample identity is shown below for each slide.


Revised region labels are shown below for each slide.


Segment labels are shown below for each slide.


Ag signal is shown for single and dual vaccinated mice for each region and segment type (e.g. Lyve1+). The number of segments included is shown above each boxplot.